| Customization: | Available |
|---|---|
| Scent: | Odorless |
| Moisture: | ﹤10% |
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Cellulase (Food, Feed & Industrial Use)
Introduction
Habio cellulase was produced by Trichoderma species using modern genetic engineering technology, microbial fermentation technology, and advanced post-processing techniques. It is composed of multiple ingredients and can degrade cellulose in fibers to create simple sugars efficiently, thus improving the utilization of raw ingredients. It is widely used in manufacture of feed, textile, fuel ethanol, alcohol, Chinese medicine extraction, food, fruit juice-processing, etc.
Mechanism
Cellulose is a high-polysaccharides made of glucopyranose by β-1, 4-glycosidic bond. Cellulase system consists of three major components: Endoglucanases (endo-1, 4-β-D-glucanases; C1; EG, EC 3.2.1.4), cellobiohydrolases (exo-1, 4-β-D-glucanases; CX; CBH, EC 3.2.1.91), and β-glucosidases (1, 4-β-D-glucosidase; βG; BGL, EC 3.2.1.21). EG acts on insoluble cellulose surface, breaks internal bonds to disrupt the crystalline structure of cellulose and expose individual cellulose polysaccharide chains, and makes cellulose chains hydration easily. CBH cleaves 2-4 units from the ends of the exposed chains produced by EG, resulting in the tetrasaccharides or disaccharide such as cellobiose. There are two main types of CBH- one type working processively from the reducing end, and the other type working processively from the non-reducing end of cellulose. βG hydrolyses the CBH product into individual monosaccharides. Through the synergistic action of above enzyme system, cellulose can be efficiently hydrolyzed to glucose.
Definition of Activity
One unit of cellulose is defined as the quantity of enzyme, which liberates 1μmol of reducing sugar from 4.0mmol/L sodium carbixymethyl cellulose substrate at 37°C and pH5.5 in 1min.
Product Functions
Feed Industry: Degrading cellulose to cello-oligosaccharide specifically, decreasing the chyme mucosity. Breaking down the structure of cell walls and releasing the nutrients inside, increasing the diffusion rate of endogenous digestive enzymes and enhancing the nutrients digestibility. Balancing intestinal flora to enhance animal immunity and reduce diarrhea ratio. Improving the animal production performance to increase customer profits.
Fabric and Textiles: Improving the finish, sheen, flexibility, air-permeability, insulation and softness of the textile fabrics, developing permanent resistance of fabric to lint, fuzz and pill buildups and easing further treatments and dye processes.
Breweries and Wineries: Breaking down plant cell walls and membranes, boosting the release of starch, and in turn, the output of alcohol.
Food, Chinese medicine, etc.: Improving the effectiveness, absorption and nutritional effects of product.
Product features
Full -scale enzyme profile (EG, CBH and βG) ensures the efficient degradation of cellulose.
Optimal genetic engineering strain, advanced production technology of liquid submerged.
Fermentation and unique post-processing lead high cellulase activity and good stability.
Broad enzyme profile, high enzyme activity and good thermostability help it more suitable for varieties of processing.
Acid Cellulase for Bio-polishing
INTRODUCTION:
Cellulose is an unbranched glucose polymer composed of anhydro-D-glucose units linked by 1, 4-b-D-glucoside bonds. These glycosidic bonds can be hydrolysed by cellulolytic enzymes. The native structure of the cellulose is composed of crystalline and less organized amorphous regions. It is an enzyme preparation obtained from the submerged fermentation of a high cellulase-producing microbial strain. It is widely used in textile and garment stone wash industries.
WORKING MECHANISM:
The 1, 4-b-D-glucosidic linkages in cellulose, lichenin and cereal b-D glucans are hydrolyzed to release free glucose units through the successive action of cellulase enzymes.
The less organized amorphous region at the center of the cellulose chain is initially attacked by endoglucanase by random cutting of the b-1, 4 glycosidic linkages within the chain thereby producing cello-oligosaccharides. Cellobiohydrolases I and II act on the cello-oligosaccharides from the reducing end and non-reducing end of the cello-oligosaccharides to release cellobiose. b- glucosidase act on the released cellobiose to produce b-D glucose units.
PROPERTIES:
It will function from a pH of 4.5 to 5.5; with pH 4.8 as an optimum. It will function from 45ºC to 60ºC and optimum temperature is 55ºC.
Appearance: Cream to white powder (Note that color does not affect or reflect activity.)
Odor: Slight fermentation odor
pH (1% soln):3.5-5.5
SPECIFICATION:
High activity product is available upon request of our customers.
ENZYME ACTIVITY UNIT DEFINITION:
Reducing sugar method: At a pH of 4. 8 and temperature of 50 ºC, to produce the amount of reducing sugar equivalent to 1 mg glucose, it needs l g solid enzyme (or 1mL liquid enzyme) and 1 h hydrolyze sodium carboxymethyl cellulose substrate, as 1 enzyme activity unit, state as u/g (or u/mL). CMCA - DNS for short.
EXECUTION STANDARD:
Light industry standard of the People's Republic of China
USAGE:
It is a kind of acidic solid cellulase, used for biofinishing, significantly soften 100% cotton fabrics, both woven and knits.
- provide soft touch except used for jean leaven dye processing.
- can obviously improve clothing surface magnitude.
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